Year: 2013 | Month: December | Volume 2 | Issue 2
Study of Cytomegalovirus Antigen Detection in Patients under Hemodialysis
Abstract: <div>Cytomegaloviral infection is an important cause of disease in immunocompromised patients e.g chronic renal failure. Laboratoty diagnosis of active stage of infection remains problem if based upon serological tests. Therefore detection of CMV antigens in peripheral leucocytes by immunohistochemical stain and PCR are mandatory in diagnosis of CMV infection dialysis patients.In this study 29 dialysis patients were assessed for the presence of cytomegalovirus by direct immunohistochemical staining of early CMV antigen (P65) in blood leucocytes, quantitative PCR detection of DNA in blood and by serology (IgG,IgM). CMV antigen was detected in 14 (48.3%) of patients, CMV PCR was positive in 10 (34.5%) patients, IgG was positive in 21 (72%) and 5 (17%) were positive for IgM. There was significant correlation (P<.0001) between PCR method and direct</div><div>staining of CMV early antigen (P65) in peripheral leucocytes.</div><div>These results suggest that direct detection of CMV antigen in peripheral leucocytes is as sensitive as PCR technique. Antigen detection is more valuable than serology in actual diagnosis of active CMV infection.</div>
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